Engineering a thermostable transketolase for arylated substrates
نویسندگان
چکیده
منابع مشابه
Second generation engineering of transketolase for polar aromatic aldehyde substrates.
Transketolase has significant industrial potential for the asymmetric synthesis of carboncarbon bonds with new chiral centres. Variants evolved on propanal were found previously with nascent activity on polar aromatic aldehydes 3-formylbenzoic acid (3-FBA), 4-formylbenzoic acid (4-FBA), and 3-hydroxybenzaldehyde (3-HBA), suggesting a potential novel route to analogues of chloramphenicol. Here w...
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The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for...
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ژورنال
عنوان ژورنال: Green Chemistry
سال: 2017
ISSN: 1463-9262,1463-9270
DOI: 10.1039/c6gc02017h